Oral vaccination against diphtheria using polyacryl starch microparticles as adjuvant.

Mucosal vaccination against diphtheria using starch microparticles as adjuvant for cross-reacting material (CRM197) of diphtheria toxin.

Mucosal vaccination has the advantage of eliciting a local mucosal immune response as well as a systemic response. In this investigation, polyacryl starch microparticles were conjugated to diphtheria toxin cross-reacting material (CRM197) as a mucosal adjuvant for oral or intranasal immunisation of mice. Various methods of stabilising CRM197 with formaldehyde were investigated. A good systemic ...

Extracellular antigens from Salmonella enteritidis induce effective immune response in mice after oral vaccination.

We have studied polyacryl starch microparticles as an adjuvant in oral vaccination in mice. Secreted antigens from Salmonella enterica serovar Enteritidis were administered covalently conjugated to microparticles, or as free antigens, orally or intramuscularly and evaluated for their immunogenicity and ability to elicit protective immune response against an oral challenge with live serovar Ente...

Starch Microparticles as an Oral Vaccine Adjuvant with Emphasis on the Differentiation of the Immune Response

Preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery

  During last two decades, polysaccharides such as alginate (Alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. The aim of the present work was to investigate the potential utility of microparticles made of alginate (Alg) as new vehicles for improving nasal vaccine delivery. For this purpose, diphtheria toxoid (DT) was chosen as a model an...

Study of the immune adjuvant effect of chitin microparticles on plasma levels of IgG1 and IgG2a during immunization against Leishmania infection in BALB/c mice

Background: There are reports on the ability of chitin microparticles to modulate the TH1 and TH2 responses, depending on the size and administration route. The purpose of the present study was to investigate the immunoadjuvant effects of the small-sized (less than 40 microns) chitin microparticles (CMP) in vaccination against Leishmania major for preventing leishmaniasis in BALB /c mice by ...